Integrative response of arsenic uptake, speciation and detoxification by Salix atrocinerea.
Identifieur interne : 000460 ( Main/Exploration ); précédent : 000459; suivant : 000461Integrative response of arsenic uptake, speciation and detoxification by Salix atrocinerea.
Auteurs : Alejandro Navazas [Espagne] ; Sophie Hendrix [Belgique] ; Ann Cuypers [Belgique] ; Aida González [Espagne]Source :
- The Science of the total environment [ 1879-1026 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Feuilles de plante, Racines de plante.
- métabolisme : Arsenic, Polluants du sol, Salix.
- Dépollution biologique de l'environnement, Inactivation métabolique, Transport biologique.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Arsenic, Soil Pollutants.
- chemistry : Plant Leaves, Plant Roots.
- metabolism : Salix.
- Biodegradation, Environmental, Biological Transport, Inactivation, Metabolic.
Abstract
Despite arsenic (As) being very toxic with deleterious effects on metabolism, it can be tolerated and accumulated by some plants. General genetic mechanisms responsible for As tolerance in plants, including Salix species, have been described in transcriptomic analysis, but further experimental verification of the significance of particular transcripts is needed. In this study, a Salix atrocinerea clone, able to thrive in an As-contaminated brownfield, was grown hydroponically in controlled conditions under an As concentration similar to the bioavailable fraction of the contaminated area (18 mg kg-1) for 30 days. At different time points, i.e. short-term and long-term exposure, biometric data, As accumulation, phytochelatin synthesis, non-protein thiol production and expression of target genes related to these processes were studied. Results showed that S. atrocinerea presents a great tolerance to As and accumulates up to 2400 mg As kg-1 dry weight in roots and 25 mg As kg-1 dry weight in leaves. Roots reduce As V to As III rapidly, with As III being the predominant form of As accumulated in root tissues, whereas in the leaves it is As V. After 1 d of As exposure, roots and leaves show de novo synthesis and an increase in non-protein thiols as compared to the control. Integrating these data on As accumulation in the plant and its speciation, non-protein thiol production and the kinetic gene expression of related target genes, a fundamental role is highlighted for these processes in As accumulation and tolerance in S. atrocinerea. As such, this study offers new insights in the plant tolerance mechanisms to As, which provides important knowledge for future application of high-biomass willow plants in phytoremediation of As-polluted soils.
DOI: 10.1016/j.scitotenv.2019.06.279
PubMed: 31279189
Affiliations:
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Electronic address: alejandro.navazas@uhasselt.be.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium; Department of Organisms and Systems Biology, Area of Plant Physiology, University of Oviedo, Catedrático Rodrigo Uría s/n, 33006 Oviedo</wicri:regionArea><placeName><region nuts="2" type="communauté">Principauté des Asturies</region></placeName></affiliation></author><author><name sortKey="Hendrix, Sophie" sort="Hendrix, Sophie" uniqKey="Hendrix S" first="Sophie" last="Hendrix">Sophie Hendrix</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium. Electronic address: sophie.hendrix@uhasselt.be.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek</wicri:regionArea><wicri:noRegion>B-3590 Diepenbeek</wicri:noRegion></affiliation></author><author><name sortKey="Cuypers, Ann" sort="Cuypers, Ann" uniqKey="Cuypers A" first="Ann" last="Cuypers">Ann Cuypers</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium. Electronic address: ann.cuypers@uhasselt.be.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek</wicri:regionArea><wicri:noRegion>B-3590 Diepenbeek</wicri:noRegion></affiliation></author><author><name sortKey="Gonzalez, Aida" sort="Gonzalez, Aida" uniqKey="Gonzalez A" first="Aida" last="González">Aida González</name><affiliation wicri:level="1"><nlm:affiliation>Department of Organisms and Systems Biology, Area of Plant Physiology, University of Oviedo, Catedrático Rodrigo Uría s/n, 33006 Oviedo, Spain; Institute of Biotechnology of Asturias, Spain. Electronic address: aidag@uniovi.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Organisms and Systems Biology, Area of Plant Physiology, University of Oviedo, Catedrático Rodrigo Uría s/n, 33006 Oviedo, Spain; Institute of Biotechnology of Asturias</wicri:regionArea><wicri:noRegion>Spain; Institute of Biotechnology of Asturias</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Science of the total environment</title><idno type="eISSN">1879-1026</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arsenic (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Biological Transport (MeSH)</term><term>Inactivation, Metabolic (MeSH)</term><term>Plant Leaves (chemistry)</term><term>Plant Roots (chemistry)</term><term>Salix (metabolism)</term><term>Soil Pollutants (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arsenic (métabolisme)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Feuilles de plante (composition chimique)</term><term>Inactivation métabolique (MeSH)</term><term>Polluants du sol (métabolisme)</term><term>Racines de plante (composition chimique)</term><term>Salix (métabolisme)</term><term>Transport biologique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arsenic</term><term>Soil Pollutants</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Feuilles de plante</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Salix</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arsenic</term><term>Polluants du sol</term><term>Salix</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Biological Transport</term><term>Inactivation, Metabolic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dépollution biologique de l'environnement</term><term>Inactivation métabolique</term><term>Transport biologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Despite arsenic (As) being very toxic with deleterious effects on metabolism, it can be tolerated and accumulated by some plants. General genetic mechanisms responsible for As tolerance in plants, including Salix species, have been described in transcriptomic analysis, but further experimental verification of the significance of particular transcripts is needed. In this study, a Salix atrocinerea clone, able to thrive in an As-contaminated brownfield, was grown hydroponically in controlled conditions under an As concentration similar to the bioavailable fraction of the contaminated area (18 mg kg<sup>-1</sup>) for 30 days. At different time points, i.e. short-term and long-term exposure, biometric data, As accumulation, phytochelatin synthesis, non-protein thiol production and expression of target genes related to these processes were studied. Results showed that S. atrocinerea presents a great tolerance to As and accumulates up to 2400 mg As kg<sup>-1</sup> dry weight in roots and 25 mg As kg<sup>-1</sup> dry weight in leaves. Roots reduce As V to As III rapidly, with As III being the predominant form of As accumulated in root tissues, whereas in the leaves it is As V. After 1 d of As exposure, roots and leaves show de novo synthesis and an increase in non-protein thiols as compared to the control. Integrating these data on As accumulation in the plant and its speciation, non-protein thiol production and the kinetic gene expression of related target genes, a fundamental role is highlighted for these processes in As accumulation and tolerance in S. atrocinerea. As such, this study offers new insights in the plant tolerance mechanisms to As, which provides important knowledge for future application of high-biomass willow plants in phytoremediation of As-polluted soils.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31279189</PMID><DateCompleted><Year>2019</Year><Month>11</Month><Day>26</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1026</ISSN><JournalIssue CitedMedium="Internet"><Volume>689</Volume><PubDate><Year>2019</Year><Month>Nov</Month><Day>01</Day></PubDate></JournalIssue><Title>The Science of the total environment</Title><ISOAbbreviation>Sci Total Environ</ISOAbbreviation></Journal><ArticleTitle>Integrative response of arsenic uptake, speciation and detoxification by Salix atrocinerea.</ArticleTitle><Pagination><MedlinePgn>422-433</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0048-9697(19)32852-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.scitotenv.2019.06.279</ELocationID><Abstract><AbstractText>Despite arsenic (As) being very toxic with deleterious effects on metabolism, it can be tolerated and accumulated by some plants. General genetic mechanisms responsible for As tolerance in plants, including Salix species, have been described in transcriptomic analysis, but further experimental verification of the significance of particular transcripts is needed. In this study, a Salix atrocinerea clone, able to thrive in an As-contaminated brownfield, was grown hydroponically in controlled conditions under an As concentration similar to the bioavailable fraction of the contaminated area (18 mg kg<sup>-1</sup>) for 30 days. At different time points, i.e. short-term and long-term exposure, biometric data, As accumulation, phytochelatin synthesis, non-protein thiol production and expression of target genes related to these processes were studied. Results showed that S. atrocinerea presents a great tolerance to As and accumulates up to 2400 mg As kg<sup>-1</sup> dry weight in roots and 25 mg As kg<sup>-1</sup> dry weight in leaves. Roots reduce As V to As III rapidly, with As III being the predominant form of As accumulated in root tissues, whereas in the leaves it is As V. After 1 d of As exposure, roots and leaves show de novo synthesis and an increase in non-protein thiols as compared to the control. Integrating these data on As accumulation in the plant and its speciation, non-protein thiol production and the kinetic gene expression of related target genes, a fundamental role is highlighted for these processes in As accumulation and tolerance in S. atrocinerea. As such, this study offers new insights in the plant tolerance mechanisms to As, which provides important knowledge for future application of high-biomass willow plants in phytoremediation of As-polluted soils.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Navazas</LastName><ForeName>Alejandro</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium; Department of Organisms and Systems Biology, Area of Plant Physiology, University of Oviedo, Catedrático Rodrigo Uría s/n, 33006 Oviedo, Spain. Electronic address: alejandro.navazas@uhasselt.be.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hendrix</LastName><ForeName>Sophie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium. Electronic address: sophie.hendrix@uhasselt.be.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cuypers</LastName><ForeName>Ann</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium. Electronic address: ann.cuypers@uhasselt.be.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>González</LastName><ForeName>Aida</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Organisms and Systems Biology, Area of Plant Physiology, University of Oviedo, Catedrático Rodrigo Uría s/n, 33006 Oviedo, Spain; Institute of Biotechnology of Asturias, Spain. Electronic address: aidag@uniovi.es.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>06</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Sci Total Environ</MedlineTA><NlmUniqueID>0330500</NlmUniqueID><ISSNLinking>0048-9697</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>N712M78A8G</RegistryNumber><NameOfSubstance UI="D001151">Arsenic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001151" MajorTopicYN="N">Arsenic</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008658" MajorTopicYN="N">Inactivation, Metabolic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032108" MajorTopicYN="N">Salix</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arsenic</Keyword><Keyword MajorTopicYN="N">Gene expression</Keyword><Keyword MajorTopicYN="N">Non-protein thiols</Keyword><Keyword MajorTopicYN="N">Phytochelatins</Keyword><Keyword MajorTopicYN="N">Salix</Keyword><Keyword MajorTopicYN="N">Speciation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>04</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>06</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31279189</ArticleId><ArticleId IdType="pii">S0048-9697(19)32852-9</ArticleId><ArticleId IdType="doi">10.1016/j.scitotenv.2019.06.279</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li><li>Espagne</li></country><region><li>Principauté des Asturies</li></region></list><tree><country name="Espagne"><region name="Principauté des Asturies"><name sortKey="Navazas, Alejandro" sort="Navazas, Alejandro" uniqKey="Navazas A" first="Alejandro" last="Navazas">Alejandro Navazas</name></region><name sortKey="Gonzalez, Aida" sort="Gonzalez, Aida" uniqKey="Gonzalez A" first="Aida" last="González">Aida González</name></country><country name="Belgique"><noRegion><name sortKey="Hendrix, Sophie" sort="Hendrix, Sophie" uniqKey="Hendrix S" first="Sophie" last="Hendrix">Sophie Hendrix</name></noRegion><name sortKey="Cuypers, Ann" sort="Cuypers, Ann" uniqKey="Cuypers A" first="Ann" last="Cuypers">Ann Cuypers</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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